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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Cancer Res. 2008 Aug 1;68(15):6136–6144. doi: 10.1158/0008-5472.CAN-08-0464

Figure 3. MUC1-C attenuates caspase-8 activation in the response of MCF-10A cells to death receptor stimulation.

Figure 3

A-C. MCF-10A cells were transfected with control siRNA or MUC1 siRNA pools for 72 h and then stimulated with TRAIL (A), TNFα (B) or FasL (C). Lysates were immunoblotted with the indicated antibodies (left panels). Lysates were also assayed for caspase-8 activity using the BD ApoAlert kit (right panels). The results are expressed as the absorbance (Abs) at 405 nm. D. Lysates from MCF-10A cells left untreated or stimulated with 100 ng/ml TRAIL for the indicated times were immunoprecipitated with anti-caspase-8. The precipitates were immunoblotted with the indicated antibodies.