Skip to main content
. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Cancer Res. 2008 Aug 1;68(15):6136–6144. doi: 10.1158/0008-5472.CAN-08-0464

Figure 4. MUC1-CD binds directly to caspase-8-p18.

Figure 4

A. Amino acid sequence of MUC1-CD with highlighting of phosphorylation sites and regions for β-catenin, IKKβ and IKKγ binding (upper panel). GST or GST-caspase-8 was incubated with purified His-MUC1-CD. The adsorbates and the input protein were immunoblotted with anti-His and anti-GST (lower panels). B. Schema of caspase-8 highlighting the N-terminal region containing the DEDs, and the p18 and p10 fragments (upper panel). GST and the indicated GST-caspase-8 fragments were incubated with His-MUC1-CD. The adsorbates and input protein were immunoblotted with anti-His and anti-GST (lower panels). C. Schema of the His-caspase-8-p18 fragment and the A, B, C and AB subfragments (upper panel). The shaded region denotes position of the His tag. GST or GST-MUC1-CD was incubated with the indicated His-caspase-8 proteins. The adsorbates and input proteins (1/10th that used in the reactions) were immunoblotted with anti-His and anti-GST (lower panels). D. GST or the indicated GST-MUC1-CD proteins were incubated with His-caspase-8-p18 (upper panel). The adsorbates were immunoblotted with anti-His and anti-GST. GST or GST-MUC1-CD(1-20) was incubated with His-caspase-8-p18 in the presence of increasing amounts of MUC1-CD(1-20) peptide (lower panel). The adsorbates were immunoblotted with anti-caspase-8. Input of the GST proteins was assessed by Coomassie blue staining.