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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Cancer Res. 2008 Aug 1;68(15):6136–6144. doi: 10.1158/0008-5472.CAN-08-0464

Figure 6. MUC1-CD competes with caspase-8 for direct binding to the FADD DED.

Figure 6

A. GST and the indicated GST-MUC1-CD proteins were incubated with purified FADD. The adsorbates and input FADD were immunoblotted with anti-FADD. The GST and GST-MUC1-CD proteins were stained with Coomassie blue. B. Schematic representation of full length FADD, N-FADD and C-FADD with positioning of the DED and DD (upper panel). The indicated GST or GST-FADD proteins were incubated with purified MUC1-CD. The adsorbates and input MUC1-CD were immunoblotted with anti-MUC1-C (lower panel). Input of the GST and GST-FADD proteins was assessed with Coomassie blue staining. C. GST or GST-caspase-8(1-183) containing the DEDs was incubated with FADD in the absence or presence of increasing amounts of purified MUC1-CD. The adsorbates and input FADD were immunoblotted with anti-FADD. Input of GST and GST-caspase-8(1-183) was determined by Coomassie blue staining. Input of MUC1-CD was determined by immunoblotting. D. Proposed interactions of MUC1-C with FADD and caspase-8 in blocking death receptor signaling.