Skip to main content
. Author manuscript; available in PMC: 2009 Jul 15.
Published in final edited form as: J Immunol. 2008 Jul 15;181(2):1548–1555. doi: 10.4049/jimmunol.181.2.1548

Figure 2.

Figure 2

Tat diminishes RON expression in monocyte/macrophages. A) U937-RON cells were treated without and with 50 ng/ml of recombinant Tat for 24 h. Cells were lysed and immunoblots were performed using anti-RON polyclonal antibody. B) Primary monocyte-derived-macrophages transduced with MSCV-RON were incubated with 100 ng/ml recombinant Tat for 24 h. Cells were lysed and immunoblotting was performed using anti-RON polyclonal antibody. C) Primary mouse peritoneal macrophages were treated with 100 ng/ml recombinant Tat for 24 h. Cells were lysed and immunoblots were performed using anti-Ron (RON) antibody. D) U937-RON cells were infected with HIV clones Mtat30 or HXB.2. Controls included uninfected cells (Uni). 24 h post-infection, immunoblots were performed on whole cell extract to determine RON expression. For these experiments the immunoblots were stripped and reprobed for β-actin. RON expression relative to β-actin was assessed by densitometry. All experiments were performed a minimum of three times. All composite figures were taken from the same gel and a single exposure.