Figure 1.

Figure 1a. Trichrome stained renal sections from mice maintained on a low salt diet for five weeks and treated with vehicle, CyA or CyA + an IL-6 neutralizing antibody.

Figure 1b. Quantification of tubular necrosis from Trichrome stained slides from treated animals. Slides were blinded and ten photographs of cortical areas per treatment group were taken, which covered an estimated 10% of the surface area in a random fashion. Areas of renal damage, which were determined by tubular necrosis, were identified and quantified by amount of necrosis per photograph. Each photograph was divided into ten blocks, and the number of blocks that contained necrotic tubules per photo was recorded. Data is represented as average block damage per all images and was anlysed by performing an ANOVA, p= 0.027.