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A: NES-B3T cells have higher levels of MEK1 phosphorylation at an inhibitory site than NES-G2T cells. Total MEK1/2 and β-tubulin were used as loading controls. B: acid exposure increases MEK1/2 activity (measured as ERK1/2 phosphorylation) in NES-G2T but not in NES-B3T cells. β-tubulin expression in whole cell lysates served as a control to confirm that equal amounts of protein were added in the immunoprecipitation reaction.
