Fig. 3.

A: transferrin receptor-1 (TfR1) and hemochromatosis gene (HFE) mRNA levels are increased in Alc-KC. TfR1, TfR2, and HFE mRNA levels were analyzed for Alc-KC and Cont-KC as described for Fig. 2A. *P < 0.05 compared with Cont-KC. B: Alc-KC express higher levels of TfR1 protein as determined by immunoblot analysis. C: Alc-KC have significantly higher uptake of Fe⁵⁹Cl₃ compared with Cont-KC. The cells isolated from the animals were incubated with Fe⁵⁹Cl₃ in serum-containing media for the indicated periods, and the radioactivity in the cells was counted after washing. *P < 0.05 compared with Cont-KC.