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. 2008 Jul 17;295(3):G442–G451. doi: 10.1152/ajpgi.90280.2008

Fig. 5.

Fig. 5.

LC8 is associated with a component of serine847-phosphorylated nNOS. Varicosity extracts were immunoprecipitated with LC8 antibody, and the immunoprecipitate and the supernatants were probed with anti-serine847-P-nNOS antibody. The LC8 immunoprecipitate showed 3 phospho-nNOS bands (A), whereas the supernatant also showed a 320-kDa phospho-nNOS (B). Varicosity extract immunoprecipitated with serine847-P-nNOS antibody and probed with anti-LC8 antibody showed a 10-kDa signal (C). Appropriate IgG controls are shown.