Fig. 6.
Membrane-bound nNOS isoforms do not bind with LC8. A: the membrane fraction of the varicosities showed only a 320-kDa band when probed with anti-nNOS1422–1433 antibody. Purity assessment was done by immunodetection of nerve membrane (synaptophysin) and cytosol [lactate dehydrogenase (LDH)]-specific markers. B: CaM-IP probed with total nNOS antibody showed 320-kDa band that did not react with serine847-P-nNOS antibody. The supernatant obtained after CaM-IP also showed a 320-kDa band that reacted with serine847-P-nNOS antibody. C: LC8-IP probed with total nNOS antibody did not show any signal.