Fig. 3.

RT-PCR and Western blot detected the presence of PGR in freshly dissociated muscle cells (controls) and in cells cultured in tissue medium with empty vector or transfection buffer or transfected with plasmids containing PGR-A, PGR-B, and PGR-AB. A: RT-PCR shows an increase in PGR-to-GADPH ratios of PGR-A, PGR-B, and PGR-AB in muscle cells transfected with PGR-A, PGR-B, and PGR-AB compared with all control cells (*P < 0.02, **P < 0.01, #P < 0.001, determined by ANOVA). B: PGR-B-to-β-actin OD ratios determined by Western blot show an increase in the protein expression of PGR-B in muscle cells transfected with plasmid DNA of PGR-B or PGR-AB compared with controls or cells transfected with PGR-A (*^,**P < 0.01 determined by ANOVA). Values are means ± SE; n = 3/group.