Table 1.

Changing ratio of polyA-primed to random-primed RNA in RAW264.7 lysates with the addition of recombinant TTP

	Time
	0 min	5 min	15 min	30 min
Lysate alone	1.0	1.1	1.1	0.9
Lysate + TTP	1.0	0.58	0.45	0.17

The Sybr-Green real-time PCR results in Fig. 2E were normalized to the input RNA by the cycle threshold method, then the ratio between the polyA-primed vs. random-primed RNA calculated at each time point. The poly(A) dependence of the tristetraprolin (TTP) effect is seen as the ratio changes with increasing time.