Fig. 5.

Enhanced ET-1-induced pulmonary VSM Ca²⁺ sensitization following CH is mediated by Rho kinase (ROK) but not PKC or tyrosine kinase. Percent vasoconstriction to ET-1 (10⁻¹⁰ to 10⁻⁷ M) in Ca²⁺-permeabilized, endothelium-disrupted small pulmonary arteries from control (A, C, E, and G) and CH (B, D, F, and H) rats in the presence of ML-9 (100 μM; A and B), Y-27632 (10 μM; C and D), GF-109203X (1 μM; E and F), genistein (100 μM; G and H), or vehicle [PSS, DMSO, or 50% ethanol (EtOH) as indicated]. Values are means ± SE. *P ≤ 0.05 vs. control group. #P ≤ 0.05 vs. corresponding vehicle group. n = 5 per group for all treatments. For purposes of illustration, the same vehicle group is used in C/E and D/F. Vehicle groups in A–F are additionally the same as those shown in Figs. 3 and 4.