Table 1.

Primers for PCR of ephrins

Target	Primer		Annealing Temp, °C
	Forward	Reverse
	Bovine
Ephrin-a1	CCTGGAATCATCTGTCCTCATTACG	AAACCCTGTGAAGCGGTGGAAC	57
Ephrin-a2	ACTGGTGAAGATGGGCTCTGG	TCCCTGGGCTTTGAGTTCCG	57
Ephrin-a5	GATGTTGACGCCGCTGTTTC	TCCCACCTCTTGAACCCTTTGG	57
EphA1	TTTGGGGAAGTGTATCGGGG	AAGGCTCCATTCTCCATAAACTCC	57
EphA2	ACCCTGGCTGATTTTGACCC	ATCCTCTTGATGTCGTCGTTGG	59
EphA3	GCCAGCGATGTATGGAGTTACG	GGGTCTGTTGTTTCTGTCTTTCTGC	56
EphA4	GAACAACTGGCTGCGAACTGAC	AGCACCCACATCCTGAAAAGC	50
EphA5	GCTGACACTGGTGGAAGGAAAG	CATTACCTGCCTCAATCTCAAAGG	55
EphA6	CTGAAATGGATGAACACAACAGGC	ACCAAGGAGGAAGAATCAACCC	55
	Human
Ephrin-a1	CAAAATCACTCACAGTCCTCAGGC	GAGCGATGCTATGTAGAACCCG	57
EphA2	TGCCAGTGTCAGCATCAACCAG	AGTCTCCCTTCTTGCGGTAAGTG	59
EphA5	TGACCTTGGAGGATTTGAGACG	TGGCACCATTCCGTTTACCAGC	55
EphA6	TGGAGCCATTCTGGACTACGAG	AGACCTGTGATGATGACACTGGG	53