FIG. 5.

OHCs and many IHCs are lost. Immunofluorescence for parvalbumin (green) labeling in the apical region of a cochlea taken from an animal injected once with kanamycin and once with furosemide and allowed to recovery for 7 days (7d). Nuclei were counterstained with propidium iodide (PI, red). An epifluorescence image of the whole-mount preparation is shown along with a confocal image of the boxed area (inset). The confocal image is a brightest point projection of a Z series stack spanning the full HC and much of the SC layers. OHCs are missing entirely from the apical turn, and scattered IHCs remain. Scale bar = 200 and 20 μm for the body of the figure and inset, respectively.