Table 1.

Tissues collected postmortem, assays performed, and tissue function

Tissue	Assay	Function
Extensor digitorum longus	In vitro contractile and mechanical properties	Toe extension and foot dorsiflexion
	Total protein content (BCA)
	Contractile protein content (SDS-PAGE)
	MyHC isoform distribution (SDS-PAGE)
Quadriceps	Metabolic activity (citrate synthase)	Knee extension
	Metabolic activity (β-HAD)
Soleus	Metabolic activity (citrate synthase)	Foot plantar flexion
Gastrocnemius	Lipid peroxidation (MDA assay)	Foot plantar flexion
Heart	Lipid peroxidation (MDA assay)	Blood circulation
	Metabolic activity (citrate synthase)
	Metabolic activity (β-HAD)
Blood	Antioxidant capacity (Trolox assay)	O2/CO2, nutrient, and metabolite transport, etc.
	Creatine kinase activity

MyHC, myosin heavy chain; β-HAD, β-hydroxy acyl-CoA dehydrogenase.