Table 1.

Hypothalamic PTP1B protein levels, hypothalamic SOCS-3 mRNA levels, and BAT UCP1 protein levels at death from 3- and 30-mo-old rats following chow or HF feeding

	Young (3 mo old)		Aged (30 mo old)
	Chow Fed	HF Fed	Chow Fed	HF Fed
PTP1B protein	1.00±0.04	1.25±0.06	1.31±0.04*	1.27±0.09
SOCS-3 mRNA	1.00±0.04	1.27±0.09†	1.20±0.04	1.41±0.08
BAT UCP1	1.00±0.14	2.53±0.40‡	1.30±0.25	3.19±0.48‡

Values are means ± SE in arbitrary units; n = 3–9 rats/group. Levels in young chow-fed rats are set to 1.0 with SE adjusted accordingly. HF, high fat; PTP1B, protein tyrosine phosphatase 1B; SOCS-3, suppressor of cytokine signaling-3; BAT, brown adipose tissue; UCP1, uncoupling protein 1. PTP1B: P = 0.017 for difference with HF feeding and P = 0.038 for difference with age by two-way ANOVA.

^*P < 0.05 for the difference between chow-fed 3- and 30-mo-old rats by post hoc analysis. SOCS-3: P = 0.006 for difference with HF feeding and P = 0.022 for difference with age by two-way ANOVA.

^†P < 0.05 for the difference between 3-mo-old HF-fed and 3-mo-old chow-fed rats. BAT UCP-1: P < 0.0001 for the difference with HF feeding by two-way ANOVA.

^‡P < 0.01 for the difference with HF feeding in both 3- and 30-mo-old rats by post hoc analysis.