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Renal SP cells can form hollow “spheroids” in 3D culture. SP and NSP renal cells from NK and RCC were cultured suspended in Matrigel in the presence of an irradiated STO layer. A: 5-wk-old spheroid grown from a single SP cell in 3D culture. B: spheroids were fixed overnight in 4% formalin before paraffin embedding and sectioning. Micrographs show hematoxylin and eosin staining of spheroid sections displaying a discrete lumen surrounded by multiple layers of cells
