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Pimonidazole staining with anti-pimonidazole antibody and immunoperoxidase staining. Bound pimonidazole is shown in cortex of control (A, C, and E) and long-term, high dose l-NMMA-treated animals (B, D, and F) showing positive signals in proximal tubular profiles (A and B) and thick ascending limbs of outer medulla (C and D) and in collecting ducts of inner medulla (E and F). Original magnification ×1,000.
