Fig. 6.

p110-β inhibition does not influence Na⁺ current (A) or phosphorylation of either Akt or SGK1 (B). mpkCCD cells were grown on Transwell filters and pretreated with aldosterone and insulin or vehicle, as in Fig. 2. Cells were then treated with PIK-90 at concentrations shown, in the presence or absence of 1 μM TGX-221. After measurement of equivalent current, lysates were prepared and subjected to Western blotting as in Fig. 3.