Fig. 4.
Role of NAD(P)H oxidase on superoxide production in mTAL obtained from SS and SS.13BN rats in response to incrementing bath NaCl. A: comparison of O2− response in SS (○; n = 5) and SS.13BN (•; n = 5) mTAL of rats fed a 0.4% NaCl diet in the presence of 100 μM apocynin. Red line shows path of 0.4% NaCl-fed SS rat depicted in Fig. 1A for comparison. B, bottom: Western blot analysis of p22phox protein expression in renal outer medullary homogenates from SS (n = 6) and SS.13BN (n = 6) rats. Top: representative example of exposed film demonstrating difference in protein expression of p22phox in outer medullary samples from SS and SS.13BN rats but not in control protein β-actin. *P < 0.05 for differences between strains, as determined by Bonferroni post hoc test; γsignificant difference between treatment groups.