Fig. 5.

Fluorescence images of VEGF IR in detrusor smooth muscle in the bladder neck region in whole-mount preparations of urinary bladder in control rats (A and B) and rats treated with CYP for 4 h (C and D) and 48 h (E and F). VEGF IR in the detrusor smooth muscle increased in density with CYP-induced cystitis (4 and 48 h). Insets in A, C, and E are shown in B, D, and F, respectively. Detrusor smooth muscle nuclei were stained with YOYO-1 (yellow-green, B and F). Scale bar, 80 μm. G: increase in density of VEGF IR in detrusor smooth muscle with CYP-induced cystitis. Values are means ± SE. *P ≤ 0.01.