Fig. 4.

Immunolocalization of basal and I/R injury-induced α7nAChR subunit protein by immunohistochemistry. Representative photomicrographs show (A) staining in cortical peritubular capillaries (PTC). B: widespread expression of α7nAChR subunit protein within the endothelium of the PTC at 24 h postreperfusion. Unlabeled arrows indicate staining of the endothelium of a venule. C: relatively less extensive staining was observed in animals pretreated with nicotine. D: no staining was observed when the primary antibody was omitted or when the sections were incubated with isotype control antibodies. Original magnification, ×200.