Fig. 3.

Effect of oxidative stress on ANG II-induced phospholipase C (PLC) stimulation in renal proximal tubules from C, BSO-, T-, and BSO+T-treated rats. A: renal proximal tubular membranes were incubated with [³H]phosphatidylinositol-4,5-bisphosphate ([³H]PIP2) and challenged with indicated doses of ANG II for 10 min. The data are expressed as a percentage of stimulation produced by the indicated concentration of ANG II. A single experiment is shown, representative of 6–8 individual experiments performed in triplicate. B: membranes from control and BSO-treated rats were incubated with 3 mM deoxycholate (DOC). C: membranes from control rats were incubated with 10 pM ANG II for 10 min in the presence and absence of 1 μM Can. Bars represent means ± SE of 6–8 different experiments performed in triplicate. *P < 0.05 vs. basal using Student's t-test. #P < 0.05 vs. ANG II using 1-way ANOVA followed by post hoc Newman-Keuls multiple test.