Figure 1.

PLA₂ antagonists arrest the reassembly of the Golgi complex during recovery from IQ or BFA treatment as seen by immunofluorescence staining with the medial Golgi enzyme ManII. Control cells display a compact, interconnected Golgi complex in the juxtanuclear region of the cell (A and B). Treatment with either IQ (10 μM for 60 min) or BFA (5 μg/ml for 30 min) resulted in a diffuse Man II staining pattern (D). When IQ- or BFA-treated cells were then washed free of these drugs and incubated in normal media, Golgi complexes were fully reassembled within 1 h (E and F). However, when recovery from IQ or BFA for the same length of time was done in the presence of the PLA₂ inhibitors BEL (10 μM; G), or ONO-RS-082 (ONO, 1 μM; H), Golgi complexes reassembled only to the point of forming large, disconnected punctate structures in the juxtanuclear region of the cell. N, nucleus.