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. 1999 Jun;10(6):1763–1782. doi: 10.1091/mbc.10.6.1763

Figure 7.

Figure 7

Cells recovering from ONO-RS-082 treatment display ManII-stained tubular connections between large centrally located Golgi elements and more peripheral Golgi mini-stacks. (A) Cells were treated with BFA for 30 min, washed free of BFA, incubated with ONO-RS-082 (5 μM) for 60 min to allow reassembly up to the point of mini-stack formation, and then allowed to recover from ONO-RS-082 for 10 min before fixing for immunofluorescence. (B–D) Cells were treated with ONO-RS-082 alone (5 μM for 60 min) to generate mini-stacks and then allowed to recover in drug-free growth medium for 10 min (B and C) or 15 min (D).