Figure 1.

A time course of DNA binding to the C/EBP-binding site (APRE) of the mouse AGP-1 promoter with nuclear proteins from livers of young and aged mice treated with LPS. EMSAs were performed using 3 μg of nuclear proteins isolated from fresh livers of control (0) or LPS-injected C57BL/6 male mice aged 4 mo (A), 12 mo (B), 19 mo (C), and 28 mo (D). LPS (10 μg) was injected at time 0, and mice were killed and nuclear proteins prepared at the times indicated at the top of each lane. The double-stranded oligonucleotide corresponding to the APRE of AGP-1 promoter was labeled with [γ-³²P]ATP and T4 polynucleotide kinase. The DNA–protein complexes are indicated as C1, C2, C3, C4, and C5. Lane F contains the free DNA probe.