Figure 2.

Mobility supershift analyses of the DNA–protein complexes formed with the APRE of the AGP-1 promoter. Supershift assays were done as described in MATERIALS AND METHODS to demonstrate that the nuclear proteins involved in the formation of DNA–protein complexes with the AGP-1 APRE are members of the C/EBP family. Monospecific antibodies against synthetic peptides unique to the C/EBPα and C/EBPβ isoforms were used. Lanes F, free oligonucleotide; lanes 1, no antibody was added to the reaction mixture; lanes 2, mouse anti-C/EBPα; lanes 3, mouse anti-C/EBPβ. Nuclear extracts were prepared 0, 6, and 24 h after LPS injection as indicated. Mice 4, 19, and 28 mo of age were used. The positions of the DNA–protein complexes are noted as C1–C5; the bands formed by supershifting with C/EBPα or C/EBPβ antibody are indicated by the arrows. (A) 4 mo. (B) 19 mo. (C) 28 mo.