Figure 6.
EMSA and supershift analysis of mouse liver polysomal proteins that bind to the APRE oligonucleotide. Liver polysomes from young (A, 4-mo-old) and aged (B, 28-mo-old) control and LPS-treated mice were prepared as described in MATERIALS AND METHODS. The [32P]APRE oligonucleotide was prepared as described in MATERIALS AND METHODS. (A) Liver polysomal proteins from 4-mo-old mice; lanes 1–3, control; lane 2, anti-C/EBPα; lane 3, anti-C/EBPβ; lanes 4–6, liver polysomal proteins from 4-mo-old LPS-treated mice; lane 4, no antibody; lane 5, anti-C/EBPα; lane 6, anti-C/EBPβ. (B) Liver polysomal proteins from 28-mo-old mice; lanes 1–3, control; lane 2, anti-C/EBPα; lane 3, anti-C/EBPβ; lanes 4–6, LPS treated; lane 4, no antibody; lane 5, anti-C/EBPα; lane 6, anti-C/EBPβ; lane F, free probe.