Figure 7.

Identification of phosphopeptides generated in vivo, represented by the analysis of LAN-5 cells with tau phosphorylated with a crude fraction (see text for explanation) of cdc2. (a) 2D phosphopeptide map of tau from LAN-5 cells (same as in Figure 3a). (b) Control analysis with aliquots of the in vivo probe and of the cdc2 phosphorylated recombinant tau protein spotted onto the same plate. (c) In vitro sample of recombinant tau protein phosphorylated with cdc2 used in the control run in b. Phosphorylated residues are labeled; for identification of peptides see Table 1.