Skip to main content
. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Neurobiol Dis. 2008 May 3;31(2):242–249. doi: 10.1016/j.nbd.2008.04.008

Figure 4.

Figure 4

IRP2 knockout neurons are less vulnerable to hemoglobin. Bars represent mean culture medium LDH (± S.E.M.) in mixed neuron/astrocyte cultures treated with indicated concentrations of hemoglobin for 24 hours. LDH values are expressed as a percentage of the mean value in sister cultures exposed to 300 µM NMDA (=100), which releases essentially all neuronal LDH without injuring astrocytes. The LDH activity in sister cultures subjected to medium exchange (sham wash) only was subtracted from all values to quantify the signal specific to hemoglobin or NMDA neurotoxicity. *P < 0.05, ***P < 0.001 v. corresponding WT value, Bonferroni multiple comparisons test, n = 7–15/condition.