FIG. 2.

Isolation of the IGR-IRES-mediated translation products for N-terminal amino acid sequence analysis. (A) Plasmids for GST fusion protein expression. The open triangle indicates a T7 promoter, and open boxes indicate ORFs for CAT and GST-fused capsid protein (CP). A bold line indicates IGR-IRES. The numbers indicate nucleotide positions corresponding to the PSIV genome. Nucleotide sequences of transcripts immediately downstream of PK I and the expected N-terminal amino acid sequences are shown below. (B) Separation of GST fusion proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after isolation with glutathione-Sepharose beads. Protein bands were stained with Coomassie brilliant blue. Positions of the products are shown at left of the panel.