FIG. 3.

IGR-IRES-mediated translation in exogenous reading frames without the capsid ORF. (A) Schematic diagrams of plasmids for translation mediated by a scanning mechanism, either with or without authentic AUG triplets, and for translation mediated by IGR-IRES. Open triangles indicate T7 promoters. Open boxes indicate coding sequences to be translated. The bold line indicates IGR-IRES. The numbers above the line indicate nucleotide positions corresponding to the PSIV genome. Numbers on the left of constructs correspond to lane numbers in panel B. (B) Comparison of electrophoretic mobilities of translation products. Positions of the products are indicated at left of the panel. The arrow indicates endogenous biotinylated protein in the wheat germ extract.