TABLE 2.

Enhancing effect of 5′ region of capsid ORF on IGR-IRES-mediated translation

Construct	Capsid ORF (no. of bases)	EGFP		FLUC
		Fluorescence intensityc	Amt of protein (μg/ml)	Luminescence intensity (RLUa)	Amt of protein (μg/ml)
pT7-IRES6192-EGFP/Fluc	0	NDb	<0.20	33,416 ± 772	0.40 ± 0.01
pT7-IRES6240-EGFP/Fluc	48	1,743 ± 91	13.95 ± 0.73	314,510 ± 6,120	3.79 ± 0.08
pT7-IRES6297-EGFP/Fluc	105	2,447 ± 39	19.04 ± 0.30	417,647 ± 6,473	5.03 ± 0.08

^aRLU, relative light units.

^bND, not detectable.

^cArbitrary units.