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Lysostaphin-disrupted biofilms of MRSA strains Col and MBT 5040. Ninety-six-well polystyrene tissue culture wells (four for each sample) were inoculated with ∼108 CFU of S. aureus strain Col or MBT 5040 (as indicated). After we allowed 48 h for biofilm formation, the wells were washed twice and then incubated with (+) or without (−) 50 μg of lysostaphin/ml in PBS for 3 h. Following incubation, the wells were washed again and stained with safranin to visualize biofilms.
