Figure 6.

Characteristic electrical signals upon opening of a fusion pore between an RBC and a CV-1 cell and probability that a bound RBC did fuse. (A) The DC conductance (Y_DC) and in-phase (Y₀), and out-of-phase (Y₉₀) conductances were measured by the patch-clamp technique in a whole-cell mode, allowing the fusion pore conductance (G_P) to be calculated. The opening of a fusion pore was accompanied by a spike in DC conductance and changes in Y₀ and Y₉₀. (B) The number of current spikes in Y_DC yields the number of RBCs that have fused to the cell. By counting the number of RBCs bound to the CV-1 cell, the probability of fusion per bound RBC was obtained.