TABLE 4.

MICs of seven fluoroquinolones and penicillin G against clinical isolates of E. faecalis (EF) with defined mechanisms of resistance

Strain (no.)	Efflux	Mutant amino acida at position:					MIC (μg/ml)b
		ParC		GyrA		ParE	PEF	CIP	CIP+R	SPX	LVX	MXF	GAR	GAR+R	GEM	PEN
		85	89	84	88	453
		S	E	S	E	P
JH 2.2c	−						4	2	2	0.5	1	0.25	0.25	0.25	0.125	2
EF12	−	I					32	4	2	1	2	0.5	0.25	0.25	0.12	2
EF13	−		K				32	4	2	1	2	0.5	0.5	0.5	0.12	2
EF14 to -15 (2)	−	I			G		256	32	32	8-32	16	2-4	1	1	0.5	2-32
EF16 to -27 (12)	−	I		Y			256	32-128	32-128	32-64	32-64	4-8	2	2	0.5-2	2-4
EF30	−	I		R			256	32	32	16	32	8	2	2	1	2
EF31	−	I		K			256	128	128	16	32	4	2	2	2	2
EF32	−	I			K		256	64	64	64	32	8	2	2	1	2
EF28 to -29 (2)	−	I		Y		S	256	64-128	64-128	64	64	8	2-4	2-4	2	2
EF33 to -43 (11)	−	I		I			256	128-256	64-128	64-128	64-128	16-32	4-8	4-8	4-8	1-4
EF-44	+	I		I			256	128	32	64	64	32	8	8	8	2

^aNucleotide sequence accession numbers AB059406, AB059405, and AA081398. No mutation was found in the GyrB QRDR.

^bPEF, pefloxacin; CIP, ciprofloxacin; R, reserpine (10 μg/ml); SPX, sparfloxacin; LVX, levofloxacin; MXF, moxifloxacin; GAR, garenoxacin; GEM, gemifloxacin; PEN, penicillin G.

^cWild-type susceptible strain.