TABLE 3.
Characteristics of ESBL-producing enterobacteria isolated in general practice
| Species (total no. of isolates) | Total no. (%) of ESBL-producing isolates | Molecular typea | Locationb,c | Specimenc | Plasmid profile | β-Lactamase contentd | Antibiotypee |
|---|---|---|---|---|---|---|---|
| E. coli (1,920) | 6 (0.3) | Ec1 | C-1 | Urine | A-1 | TEM-24b | KTNtA CHL SSS TMP |
| Ec2 | NH-1 | Urine | B | TEM-21, TEM-1 | KGTNt(A) CHL SSS TMP TET NAL | ||
| Ec3 | NH-1 | Urine | B | TEM-21 TEM-1 | KGTNt(A) CHL SSS TMP TET | ||
| Ec4 | NH-1 | Urine | B | TEM-21 | KGTNt(A) CHL SSS NAL | ||
| Ec5 | C-2 | Urine | B | TEM-21, TEM-1 | KGTNt(A) CHL SSS TET OFX | ||
| Ec6 | CLI-4 | Urine | NDf | CTX-M-1, TEM-1 | CHL TET SSS | ||
| P. mirabilis (208) | 3 (1.4) | Pm1 (2) | NH-1 (2) | Pus (2) | B | TEM-21 | KGTNt(A) CHL SSS TET |
| Pm2 | NH-1 | Urine | B | TEM-21, TEM-1 | KGTNt(A) CHL SSS TET FOF OFX | ||
| K. pneumoniae (104) | 8 (7.7) | Kp1 | CLI-1 | Urine | A-2 | TEM-24b, SHV-1 | KTNt(A) CHL SSS TMP (TET) |
| Kp2 | NH-2 | Urine | A-3 | TEM-24b, SHV-1 | KTNt(A) SSS TMP NAL | ||
| Kp2 | NH-2 | Urine | A-3 | TEM-24b, SHV-1 | KTNt(A) CHL SSS TMP (TET) OFX | ||
| Kp3 | NH-1 | Urine | B | TEM-21, SHV-1 | KGTNt(A) CHL SSS | ||
| Kp4 | NH-1 | Urine | B | TEM-21, SHV-1 | KGTNt(A) CHL SSS | ||
| Kp5 | CLI-2 | Urine | ND | TEM-19, SHV-1 | KGTNt(A) SSS (TET) NAL | ||
| Kp6 | CLI-5 | Respiratory tract | ND | TEM-15, SHV-44, SHV-1, TEM-1 | GTNt SSS TMP NAL | ||
| Kp7 | C-3 | Urine | ND | TEM-15, SHV-4, SHV-1 | KTNtA CHL SSS TMP TET OFX | ||
| E. aerogenes (48) | 19 (39.6) | Ea1 (11) | CLI-1 (6), CLI-3 (2), CLI-6, NH-3, C-4 | Urine (6), respiratory tract (4), pus (1) | A-1 | TEM-24b, AmpC | KTNt(A) CHL SSS TMP (TET) OFX |
| Ea1 | CLI-2 | Urine | A-1 | TEM-24b, AmpC | KTNt(A) CHL SSS TMP (TET) OFX MOX | ||
| Ea1 (3) | CLI-1, CLI-2, CLI-6 | Respiratory tract (2), urine | A-2 | TEM-24b, AmpC | KTNt(A) CHL SSS TMP (TET) OFX | ||
| Ea1 | C-5 | Urine | A-2 | TEM-24b, AmpC | KTNt(A) CHL SSS TMP (TET) OFX MOX IPM | ||
| Ea1 | CLI-1 | Urine | A-4 | TEM-24b, AmpC | KTNt(A) CHL SSS (TET) OFX MOX IPM | ||
| Ea1 | CLI-1 | Respiratory tract | A-5 | TEM-24b, AmpC | KTNt(A) SSS (TET) OFX | ||
| Ea2 | CLI-8 | Urine | ND | TEM-3, AmpC | KTNt(A) CHL SSS TMP (TET) OFX | ||
| S. marcescens (41) | 1 (2.4) | Sm | CLI-4 | Urine | A-4 | TEM-24b, AmpC | KTNt(A) CHL SSS TMP TET OFX |
| M. morganii (36) | 1 (2.8) | Mm | CLI-1 | Urine | A-2 | TEM-24b, AmpC | KTNt(A) CHL SSS TMP TET OFX |
| P. stuartii (24) | 1 (4.2) | Ps | CLI-7 | Urine | A-1 | TEM-24b | KGTNt(A) CHL SSS TMP TET |
A single number was assigned according to the concordant results obtained by three molecular typing methods (RAPD with primer AP12h and ERIC-PCR with primers ERIC2 and ERIC1R).
The different locations are NHs, clinics (CLI), and the community (C).
The number of isolates is given in parentheses if more than one isolate was recovered.
AmpC and SHV-1, species-specific cephalosporinase and SHV-1-like chromosomal penicillinase, respectively.
The resistance cotransferred with the ESBL(s) is indicated in boldface. G, K, T, Nt, and A, gentamicin, kanamycin, tobramycin, netilmicin, and amikacin, respectively. CHL, chloramphenicol; SSS, sulfamethoxazole; TMP, trimethoprim; TET, tetracycline; NAL, nalidixic acid; OFX, ofloxacin; FOF, fosfomycin, MOX, moxalactam; IPM, imipenem. Parentheses indicate a low level of resistance.
ND, not determined.