Figure 6.

Sarkosyl removes most of large subunit of RNA polymerase II. Different numbers (1x or 1/3x) of encapsulated cells were either not extracted (total), or extracted with saponin (sap) or sarkosyl (sark). After electrophoresis and immunoblotting with 8WG16 or H5 antibodies, autoradiographs were prepared; the ∼220–240 kDa region is shown. Quantitative analysis (our unpublished results) of five pairs of blots shows the following. 8WG16 reactivity: 54% is due to IIo, saponin has little effect, and sarkosyl removes 85% IIa and 62% IIo, to leave 27% (range 22–34%) of total. H5 reactivity: saponin has little effect, but sarkosyl extracts 13% of major band.