Figure 2.

Rab2 Q65L inhibits ER-to-Golgi transport in vitro. Semi-intact NRK cells were preincubated with the indicated amount of Rab2 (WT), Rab2 Q65L, or mock-prenylated control (lacking recombinant Rab2 protein) for 30 min on ice in a transport mixture as described in MATERIALS AND METHODS. The cells were then transferred to 32°C and incubated for a total of 90 min. The fraction of VSV-G processed to the endo H–resistant forms (percent of total) was determined after analysis by SDS-PAGE and fluorography. Rab2 Q65L inhibited transport in a dose-dependent manner. Results shown are representative of three independent protein purifications.