Figure 3.

Cells incubated with Rab2 Q65L fail to transport ts045 VSV-G to the Golgi complex. NRK cells grown on coverslips were infected with ts045 VSV-G for 3 h at 39.5°C to retain ts045 VSV-G in the endoplasmic reticulum. The cells were rapidly shifted to ice, permeabilized with digitonin, and then incubated in a complete transport cocktail in the absence (Control) or presence of 100 ng of Rab2 (WT) or 100 ng of Rab2 Q65L for 40 min at 32°C. The distribution of ts045 VSV-G (left), p53/gp58 (middle), β-COP (B), and Golgi (A) staining was determined as described in MATERIALS AND METHODS. Arrows denote the alignment of costained cells. GC, Golgi complex.