Figure 4.

The inhibitory activity of Rab2 Q65L is downstream from the calphostin C–sensitive step. Semi-intact NRK cells were incubated at 32°C in a transport mixture described in MATERIALS AND METHODS. At the indicated time (Δt), the control (○) was transferred to ice, or 120 nM calphostin C (▪) or 100 ng of Rab2 Q65L (▴) was added and incubated with the cells for a total of 60 min. Transport was terminated by transfer of the cells to ice, and the fraction of VSV-G processed to endo H–resistant forms was determined as described in MATERIALS AND METHODS. Results shown are representative of three independent protein purifications.