Figure 5.

VSV-G is rapidly transported through the Rab2 Q65L–sensitive step after accumulation at 15°C. Semi-intact NRK cells were either incubated in a transport mixture for the indicated time at 32°C (□) or preincubated at a reduced temperature (15°C) for 80 min to accumulate VSV-G in pre-Golgi VTCs. Cells preincubated at 15°C were maintained at 15°C (○), shifted to 32°C and incubated for the indicated time (Δt) before transfer to ice (▪), or supplemented with 100 ng of Rab2 Q65L and incubated for a total of 80 min (▴). The fraction of VSV-G processed to endo H–resistant forms was determined as described in METHODS AND MATERIALS. Results shown are representative of three independent protein purifications