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Pyrosequencing assay to detect the G2576T mutation in LRE and linezolid-resistant staphylococci. PCR primers used to amplify a 96-bp fragment of 23S rRNA genes spanning the mutation are shaded in grey; the forward primer was biotin labeled. The pyrosequencing primer (complementary to the forward strand) is shaded in black. Nucleotide G2576 is underlined and in upper case.
