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. 1958;18(5-6):945–961.

The intradermal test in the diagnosis of bilharziasis

J Pellegrino
PMCID: PMC2537960  PMID: 13573119

Abstract

The diagnosis of bilharziasis by detection of schistosome eggs in the urine or faeces of patients is often not possible in the later stages of the disease, when elimination of the eggs is rendered increasingly difficult. To overcome this difficulty, an immunological test has been developed, based on the cutaneous response to an injection of an antigen prepared from the cercariae, adult worms, eggs or miracidia of Schistosoma.

In this paper, the author reviews the various procedures for obtaining the basic material for antigen preparation, describes briefly the method of preparation, outlines the nature and properties of the specific antigens, and discusses the skin-testing procedure, with special reference to the criteria for evaluating the results. He points out that the intradermal test may prove useful in epidemiological surveys as well as in the diagnosis of individual cases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRENER Z. Observaçes sôbre a infecço do camundongo pelo Schistosoma mansoni. Rev Bras Malariol Doencas Trop. 1956 Oct;8(4):565–575. [PubMed] [Google Scholar]
  2. CHAIA G. Tecnica para concentraço de miracïdios. Rev Bras Malariol Doencas Trop. 1956 Apr;8(2):355–357. [PubMed] [Google Scholar]
  3. CHUNG H. L., WENG H. C., HOU T. C., HO L. Y. Cross intradermal reactions of patients with paragonimiasis, clonorchiasis and schistosomiasis to different trematode antigens and their clinical significance. Chin Med J. 1955 Sep-Oct;73(5):368–378. [PubMed] [Google Scholar]
  4. CHUNG H. L., WENG H. C., HOU T. C. Immunodiagnosis and chemotherapy of Clonorchiasis sinensis, with especial reference to efficacy of chloroquine including a note on negative effect of oxychloroquine. Chin Med J. 1955 Jan-Feb;73(1):1–14. [PubMed] [Google Scholar]
  5. COKER C. M., LICHTENBERG F. A revised method for isolation of Schistosoma mansoni eggs for biological experimentation. Proc Soc Exp Biol Med. 1956 Aug-Sep;92(4):780–782. doi: 10.3181/00379727-92-22612. [DOI] [PubMed] [Google Scholar]
  6. DA SILVA J. R., DA COSTA P. D. Os testes intradérmicos e outras reaçes biológicas no diagnóstico da esquistossomiase mansoni. Med Cir Farm. 1949 Sep;71(161):497–506. [PubMed] [Google Scholar]
  7. DAVIES A. M., ELIAKIM M. The value of different antigens in the diagnosis of chronic bilharziasis by the skin and complement fixation tests. Am J Trop Med Hyg. 1954 Jul;3(4):728–741. doi: 10.4269/ajtmh.1954.3.728. [DOI] [PubMed] [Google Scholar]
  8. HORSTMAN H. A., Jr, CHAFFEE E. F., BAUMAN P. M. Schistosomiasis mansoni in Puerto Rican soldiers. Am J Trop Med Hyg. 1954 Sep;3(5):914–917. doi: 10.4269/ajtmh.1954.3.914. [DOI] [PubMed] [Google Scholar]
  9. INGALLS J. W., Jr, HUNTER G. W., 3rd The molluscan intermediate host and schistosomiasis japonica; observations on the conditions governing the hatching of the eggs of Schistosoma japonicum. J Parasitol. 1949 Apr;35(2):147–151. [PubMed] [Google Scholar]
  10. KAGAN I. G., SHORT R. B., NEZ M. M. Maintenance of Schistosomatium douthitti (Cort, 1914) in the laboratory (Trematoda: Schistosomatidae). J Parasitol. 1954 Aug;40(4):424–439. [PubMed] [Google Scholar]
  11. LURIE H. I., DE MEILLON B., EISELEN H. H. Negative cercarial antigen skin tests in proved urinary bilharziasis in children. S Afr Med J. 1953 Apr 11;27(15):295–296. [PubMed] [Google Scholar]
  12. LURIE H. I., DE MEILLON B., STOFFBERG N. Experimental bilharziasis in animals. I. Early diagnosis of bilharziasis. S Afr Med J. 1952 Dec 20;26(51):1005–1008. [PubMed] [Google Scholar]
  13. LURIE H. I., DE MEILLON B. Skin tests for schistosomiasis. S Afr Med J. 1951 May 12;25(19):321–324. [PubMed] [Google Scholar]
  14. MICHAEL DAVIES A., ELIAKIM M. Bilharzia in Israel: an immunological survey amongst recent immigrants. Ann Trop Med Parasitol. 1955 Mar;49(1):9–23. doi: 10.1080/00034983.1955.11685647. [DOI] [PubMed] [Google Scholar]
  15. MOORE D. V., YOLLES T. K., MELENEY H. E. A comparison of common laboratory animals as experimental hosts for Schistosoma mansoni. J Parasitol. 1949 Apr;35(2):156–170. [PubMed] [Google Scholar]
  16. OLIVER-GONZALEZ J., BAUMAN P. M., BENENSON A. S. Immunological aspects of infections with Schistosoma mansoni. Am J Trop Med Hyg. 1955 May;4(3):443–452. doi: 10.4269/ajtmh.1955.4.443. [DOI] [PubMed] [Google Scholar]
  17. OLIVER-GONZALEZ J., BAUMAN P. M., BENENSON A. S. Intradermal response to egg antigen in humans with active and treated (fuadin) Schistosoma mansoni infections. Proc Soc Exp Biol Med. 1954 Oct;87(1):186–188. doi: 10.3181/00379727-87-21328. [DOI] [PubMed] [Google Scholar]
  18. OLIVER-GONZALEZ J. Intradermal reactions to schistosome and filaria antigens in patients before and after treatment of the parasitic infections. Am J Trop Med Hyg. 1953 Jan;2(1):79–84. doi: 10.4269/ajtmh.1953.2.79. [DOI] [PubMed] [Google Scholar]
  19. OLIVER-GONZALEZ J., RAMOS F. L., COKER C. M. Serological reactions against egg antigens as an aid in the evaluation of therapy in schistosomiasis. Am J Trop Med Hyg. 1955 Sep;4(5):908–912. doi: 10.4269/ajtmh.1955.4.908. [DOI] [PubMed] [Google Scholar]
  20. PAN C., HUNTER G. W., 3rd A modified perfusion technique for the recovery of schistosomes. J Lab Clin Med. 1951 May;37(5):815–816. [PubMed] [Google Scholar]
  21. PELLEGRINO J., NUNES M. B. Tecnica de obtenço de cercárias dessecadas de Schistosoma mansoni para o preparo de antígenos. Rev Bras Malariol Doencas Trop. 1956 Apr;8(2):397–404. [PubMed] [Google Scholar]
  22. PELLEGRINO J., PAULINI E., MEMORIA J. M., MACEDO D. G. A reaço intradérmica, na esquistossomose, com uma fraço polissacarídea isolada de cercárias de Schistosoma mansoni. Rev Bras Malariol Doencas Trop. 1956 Jul;8(3):527–534. [PubMed] [Google Scholar]
  23. PELLEGRINO J., POMPEU MEMORIA J. M., MACEDO D. G. Quantitative aspects of the intradermal test with cercarial antigen in schistosomiasis. J Parasitol. 1957 Jun;43(3):304–307. [PubMed] [Google Scholar]
  24. PELLEGRINO J., SIQUEIRA A. F. Tecnica de perfusāo para colheita de Schistosoma mansoni em cobaias experimentalmente infestadas. Rev Bras Malariol Doencas Trop. 1956 Oct;8(4):589–597. [PubMed] [Google Scholar]
  25. PEREIRA O. A. A intradermorreaço para o diagnóstico da esquistossomose mansoni. Resen Clin Cient. 1951 Oct;20(10):331–338. [PubMed] [Google Scholar]
  26. PESIGAN T. P., GARCIA E. G., BANZON T. C., BELTRAN A. M., SANTOS A. T., ANOVER M., BASACA-SEVILLA V. Further studies on intradermal test in schistosomiasis japonica. J Philipp Med Assoc. 1954 Jan;30(1):14–22. [PubMed] [Google Scholar]
  27. PESIGAN T. P., PUTONG P. B., GARCIA E. G., MILLAR C. A. Intradermal test for schistosomiasis japonica (preliminary report). J Philipp Med Assoc. 1951 Apr;27(4):212–219. [PubMed] [Google Scholar]
  28. RUIZ J. M. Preparo do antïgeno para intradermo-reaço na esquistossomose. Mem Inst Butantan. 1953;25(1):5–13. [PubMed] [Google Scholar]
  29. RUIZ J. M. Processo rápido de perfusão do sistema porta de mamäferos para coleta de esquistossomatädeos, aplicavel aos trabalhos de campo. Mem Inst Butantan. 1953;25(2):29–33. [PubMed] [Google Scholar]
  30. RUIZ J. M. Técnica de perfusão para a coleta de Schistosoma mansoni em animais de laboratório. Mem Inst Butantan. 1952;24(2):101–109. [PubMed] [Google Scholar]
  31. SHERIF A. F. A new intradermal antigen for the diagnosis of schistosomiasis. Ann Trop Med Parasitol. 1956 Jun;50(2):105–112. doi: 10.1080/00034983.1956.11685747. [DOI] [PubMed] [Google Scholar]
  32. STIREWALT M. A., KUNTZ R. E., EVANS A. S. The relative susceptibilities of the commonly-used laboratory mammal to infection by Schistosoma mansoni. Am J Trop Med Hyg. 1951 Jan;31(1):57–82. doi: 10.4269/ajtmh.1951.s1-31.57. [DOI] [PubMed] [Google Scholar]

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