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. Author manuscript; available in PMC: 2009 Aug 28.
Published in final edited form as: J Med Chem. 2008 Aug 5;51(16):5043–5051. doi: 10.1021/jm800332m

Figure 4.

Figure 4

Measurement of 1 and 2 inhibition of HIV-1 replication using RT, p24 and Western blotting assays. MT4 cells were infected with 20,000 RT counts of virus and treated with the indicated compounds. The experiments were repeated three times. Cell supernatants were collected every 2 days and assayed for both RT activity (4A and 4B) and p24 ELISA (4C). ELISA shows a corresponding decrease in the amount of p24 present in supernatants collected from infected MT4 cultures similar to the results from the RT assays. D) 1 and 2 suppressed HIV-1 protein expression in HeLa cells transfected with HIV-1 molecular clone pNL4-3. HeLa cells were transfected with 2µg pNL4-3, and 3 hrs post-transfection the cells were treated with 25 µM concentration of 1 and 2. Cell lysates were prepared 24 hrs later and analyzed by Western blotting using anti-HIV-1 patient IgG.