Figure 3.

Computer-assisted cellular movement analysis of wild-type, ExEh20, and LMM cells. Images of amoebae were recorded and computer analyzed using conditions described in MATERIALS AND METHODS. Micrographs of analyzed amoebae are shown in the upper panel, and their trajectories are represented in the lower panel. The wild-type strain moves in random directions; some of the amoebae return on their trajectories. ExEh20 cells move at the same rate as the wild-type strain but show more direct trajectories. LMM20 and LMM30 show reduced trajectories. The majority of the amoebae wobble in place around stationary points of contact with the substratum. In addition, LMM transfectants are round, and some of them seem to extend small pseudopods.