Figure 6.

Viability of Caco-2 cells in the presence of LMM transfectants. A ⁵¹Cr-labeled, postconfluent monolayer of Caco-2 cells, cultivated in 24-well plates, was infected with growing E. histolytica (1 amoeba for 5 cells) in a 1-ml vol medium and incubated for the indicated times. After incubation, medium was removed and centrifuged. The cytolysis is reflected by the ⁵¹Cr molecules liberated in the medium. Percentage of lysis corresponds to the fraction of radioactivity in the supernatant compared with the total radioactivity. Each incubation was condition tested in triplicate, and the experiment was repeated three times. ▴—▴, HM1 wild-type strain (40% ± 3% of lysis; n = 3); ▪—▪, NEO-transfected strain: (40% ± 1% of lysis; n = 3); •—•, LMM transfected strain (5% ± 1% of lysis; n = 3).