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Creation of HDAd–prestin–GFP vector. A unique Nsi1 linker containing an Mlu1 restriction site was inserted into the pCMV-HA–Prestin–IRES–GFP plasmid. The plasmid was then linearized at the unique Mlu1 site and inserted into a compatible Asc1 restriction site in the pΔ25.3E4 HDAd vector to create HDAd–prestin–GFP vector.
