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The distribution of endobrevin is affected by wortmannin, a phosphatidylinositol 3-kinase inhibitor. After internalization of transferrin receptor antibodies for 30 min at 37°C, A431 cells were treated with 500 nM wortmannin for 30 min (A and B) and 60 min (C and D) before processing for indirect immunofluorescence microscopy. Bar, 10 μm.
