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Detection of endobrevin in the total membrane fraction prepared from MDCK II (lanes 1, 4, and 7), NRK (lanes 2, 5, and 8), and HeLa (lanes 3, 6, and 9) cells by Western blot analysis using affinity-purified antibodies against endobrevin. A 15-kDa protein was specifically detected (lanes 1–3), and the detection of this protein was blocked by preincubation of antibody with GST–endobrevin (lanes 7–9) but not by a mixture of HisX6-cellubrevin and GST (lanes 4–6).
